Aims: The aim of this study is to determine the PCE biodegradation potential in an Anaerobic Migrating Blanket Reactor (AMBR) that has not been used so far for the bioremediation of this compound, in high concentration, and to evaluate the system performance. Materials and Methods: This study was an Experimental - Interventional study that was done from April 2010 to March 2011, in the Isfahan University of Medical Sciences. The AMBR was used in a type of laboratory scale, with a volume of 10 L, which was divided into four compartments, for the biological degradation of PCE in a synthetic substrate. The startup was done using anaerobic digested sewage sludge. The performance of the reactor was evaluated during four periods, with a PCE loading rate of 3.75 until 75 mg PCE/L.d. The hydraulic retention time (HRT) was 32 hours. Results: Optimum chemical oxygen demand (COD) removal efficiency was obtained, 98%, with an organic loading rate (OLR) equal to 3.1 g COD/L.d. For PCE removal, the optimum efficiency was observed to be 99.8%, with a PCE loading rate equal to 37.5 mg PCE/L.d. The average COD and PCE removal rates for the whole activity period of the reactor were 91.4 and 99.5%, respectively; 1.1 ± 0.7% from the influent PCE was adsorbed on the biomass and 20% was found in the headspace. Conclusions: The AMBR reactor, which provides full-scale studies and uses real industrial wastewater polluted with PCE, is a simple, efficient, and reliable method for the treatment of PCE.