1
Isfahan Water and Wastewater Company, Isfahan; Science and Research Branch, Islamic Azad University, Tehran, Iran
2
Environment Research Center, Isfahan University of Medical Sciences (IUMS), Isfahan, Iran
3
Isfahan Water and Wastewater Company, Isfahan, Iran
4
Infectious Diseases and Tropical Medicine Research Center, IUMS, Isfahan, Iran
5
Science and Research Branch, Islamic Azad University, Tehran, Iran
6
Food Security Research Center, IUMS, Isfahan, Iran
Abstract
Aims: There is limited data on the occurrence of E. Coli O157: H7 in water. Therefore, this study aims to detect E. Coli O157: H7 in the Water Treatment Plant (WTP). Materials and Methods: This cross-sectional study was conducted in Isfahan WTP, central of Iran. Immunological methods were implemented with anti-serum kits and the molecular method of reverse transcription-polymerase chain reaction (RT-PCR) was used to detect E. Coli O157: H7 in eight locations of the WTP; the sludge of the sedimentation basin and filter backwash water were also monitored. The survival of E. Coli O157: H7 in the sludge samples of the sedimentation basin was indicated by the formation of agglutination particles using the immunological method, and through indicator probes using the RT-PCR method.Results: E. Coli O157: H7 was not detected in the water samples from the WTP units. The removal percent of total coliforms (TC), fecal coliforms (FC), and Heterotrophic Plate Count (HPC), respectively, were as follows: 59.5, 49, and 54.8% in the sedimentation basin; 66, 45.8, and 57% in the ozonation system; 98.8, 98, and 78.8% in the filtration system; and 96, 100, 91% in the disinfection system. Conclusions: This study revealed the existence of the pathogenic coliform of E. Coli O157: H7 in the sludge of the sedimentation basin. The absence of E. Coli O157: H7 in the finished water indicated that the WTP units were able to eliminate these pathogenic bacteria before reaching the final units of the plant, including the filtration and disinfection systems.
Atabakhsh,P , Amin,M M , Mortazavi,H , Poursafa,P , Yaran,M , Sepahi,A A , Jalali,M and Noohi,A A S . (2012). Detection of E. coli O157: H7 by immunological and real-time PCR methods in the water treatment plant. International Journal of Environmental Health Engineering (IJEHE), 1(March), 30-35.
MLA
Atabakhsh,P , , Amin,M M , , Mortazavi,H , , Poursafa,P , , Yaran,M , , Sepahi,A A , , Jalali,M , and Noohi,A A S . "Detection of E. coli O157: H7 by immunological and real-time PCR methods in the water treatment plant", International Journal of Environmental Health Engineering (IJEHE), 1, March, 2012, 30-35.
HARVARD
Atabakhsh P, Amin M M, Mortazavi H, Poursafa P, Yaran M, Sepahi A A, Jalali M, Noohi A A S. (2012). 'Detection of E. coli O157: H7 by immunological and real-time PCR methods in the water treatment plant', International Journal of Environmental Health Engineering (IJEHE), 1(March), pp. 30-35.
CHICAGO
P Atabakhsh, M M Amin, H Mortazavi, P Poursafa, M Yaran, A A Sepahi, M Jalali and A A S Noohi, "Detection of E. coli O157: H7 by immunological and real-time PCR methods in the water treatment plant," International Journal of Environmental Health Engineering (IJEHE), 1 March (2012): 30-35,
VANCOUVER
Atabakhsh P, Amin M M, Mortazavi H, Poursafa P, Yaran M, Sepahi A A, Jalali M, Noohi A A S. Detection of E. coli O157: H7 by immunological and real-time PCR methods in the water treatment plant. Int. J. Environ. Health Eng.. 2012;1(March):30-35.
International Journal of Environmental Health Engineering (IJEHE)