Aims: The biodegradability of oil containing polychlorinated biphenyls (PCBs) from electrical transformer by the anaerobic sequencing biofilm batch reactor (ASBBR) with was assessed. Materials and Methods: Two anaerobic sequencing batch biofilm reactor (ASBBR) containing polyurethane foam cubes as inert support was used. The reactors were operated for 310 days at 35 ± 2°C. The reactors with a total volume of 7 L, 5 L effective volume and 3.5 L for gas production, were operated in a cycle per day. The effect of operational parameters including organic loading rate, PCBs loading rate, co-substrate type, initial PCBs and COD concentration was evaluated. Results: The results point to admirable reactors stability and over 95% efficiency in PCBs removal, with effluent PCBs concentration of lower than 10 mg/L. However, degradation rates increased as the initial concentration of PCBs as increased. The average of COD removal efficiency by two ASBBR reactors was more than 92% that corresponding to > 9 μg/L of effluent COD. In over all operation, average of biogas production in R1 was 5.7 ± 2.2 L/d and maximum produced biogas was 8.02 L/d at 310 day. The kinetic studies revealed that second - order kinetic model described the COD removal by ASBBR reactors from synthetic wastewater better than two other kinetic model. Conclusion: Therefore, this investigation demonstrated that the ASBBR have good potential for biodegradation of oil containing PCBs, despite variation of influent PCBs and organic loading rate (OLR).