Aims: The aim of our study was to analyze the knowledge attitude and practice (KAP) of biomedical waste management among medical, paramedical students and health care professionals in Coimbatore Medical College Hospital in South India. Materials and Methods: Study was conducted among 400 health care professionals including doctors, nurses, nursing students, and laboratory technician course students. The results were evaluated. Results: It was found that the doctors had the maximum knowledge and practice among health care professionals. The results also indicate that knowledge is not uniform among individual group, and there exists considerable variation within the group. Conclusion: The results of our study revealed that the project for upgrading safety in health care initiative taken by the Government of Tamil Nadu to create awareness of biomedical waste management among health-care professionals have certainly improved the KAP on biomedical waste management. Hence, such a program is mandatory to improve the biomedical waste management in health-care centers.