1
Department of Epidemiology and Biostatistics, School of Public Health, IUMS, Isfahan, Iran
2
Food Security Research Center and Department of Microbiology, IUMS, Isfahan, Iran
3
Department of Food Hygiene, College of Veterinary Medicine, Islamic Azad University of Shahrekord Branch, Shahrekord, Iran
4
Food Security Research Center and Department of Food Science and Technology, School of Nutrition and Food Sciences, Isfahan University of Medical Sciences (IUMS), Isfahan, Iran
Aims: This study was conducted to determine the prevalence rate, virulence genes and antimicrobial resistance of Escherichiacoli O157:H7/NM isolated from hamburger and chicken nugget in Isfahan, Iran. Material and Methods: From June 2013 to July 2013, a total of 190 hamburger (120) and chicken nugget (70), were collected from four randomly selected factories in Isfahan, Iran. They were evaluated for the presence of E.coli O157:H7/NM using microbiological culture and polymerase chain reaction. Statistical analysis was performed using SPSS software version 16.0 (SPSS Inc., Chicago o, IL, USA). Results: From a total of 190 samples analyzed four samples (2.1%) were contaminated with E.coli O157. All of the E.coli O157 were isolated from hamburger samples (3.3%) and chicken nugget samples were negative. Of four E.coli O157 isolated, only one sample was serotype E.coli O157:H7 and others were serotype E.coli O157:NM. Among four E.coli O157:H7/NM isolates, one strain was positive for all stx1, stx2,eaeA and ehxA genes. One strain was positive for stx2 gene. The other two were negative for these genes. All isolates (100%) were resistant to one or more antimicrobial agents. Conclusions: The results of this study showed that hamburger could be a significant source of E.coli O157:H7 and E.coli O157:NM serotypes in Iran and multi-resistance was found in 27% of E.coli O157 strains and this is a major public health concern.
Ghasemian Safaei,H. , Jalali,M. , Mahaki,B. , Mirlohi,M. , Rahimi,E. and Miri,A. (2014). Isolation of shiga toxin-producing Escherichia coli O157:H7/NM from hamburger and chicken nugget. International Journal of Environmental Health Engineering (IJEHE), 3(August), 19-23. doi: 10.4103/2277-9183.138414
MLA
Ghasemian Safaei,H. , , Jalali,M. , , Mahaki,B. , , Mirlohi,M. , , Rahimi,E. , and Miri,A. . "Isolation of shiga toxin-producing Escherichia coli O157:H7/NM from hamburger and chicken nugget", International Journal of Environmental Health Engineering (IJEHE), 3, August, 2014, 19-23. doi: 10.4103/2277-9183.138414
HARVARD
Ghasemian Safaei H., Jalali M., Mahaki B., Mirlohi M., Rahimi E., Miri A. (2014). 'Isolation of shiga toxin-producing Escherichia coli O157:H7/NM from hamburger and chicken nugget', International Journal of Environmental Health Engineering (IJEHE), 3(August), pp. 19-23. doi: 10.4103/2277-9183.138414
CHICAGO
H. Ghasemian Safaei, M. Jalali, B. Mahaki, M. Mirlohi, E. Rahimi and A. Miri, "Isolation of shiga toxin-producing Escherichia coli O157:H7/NM from hamburger and chicken nugget," International Journal of Environmental Health Engineering (IJEHE), 3 August (2014): 19-23, doi: 10.4103/2277-9183.138414
VANCOUVER
Ghasemian Safaei H., Jalali M., Mahaki B., Mirlohi M., Rahimi E., Miri A. Isolation of shiga toxin-producing Escherichia coli O157:H7/NM from hamburger and chicken nugget. Int. J. Environ. Health Eng., 2014; 3(August): 19-23. doi: 10.4103/2277-9183.138414
International Journal of Environmental Health Engineering (IJEHE)