Aims: In this study, removal efficiency of linear alkylbenzene sulfonate (LAS) in Stabilization pond plant was investigated. Materials and Methods: In this study, 64 samples were taken for 1-year in stabilization pond plant of Yazd city, central of Iran, in 2010. The samples were analyzed according to the standard methods. Methylene blue active substances were used to determine the amount of anionic surfactants. Results: The most removal efficiency of anionic surfactants occurred in secondary facultative stabilization pond in summer and the least removal efficiency of anionic surfactant in anaerobic stabilization pond in the autumn was obtained. Conclusion: According to the environmental standards for discharge of treated wastewater to the surface water, agricultural water usage and discharge to absorbent wells with P < 0.5 have significant difference values are more than standards.