Amis: The present study was aimed to compare and evaluate the level of microbial (parasitic and bacterial) quality and physicochemical conditions of the water from five different types of indoor swimming pools (three men's and two women's pools). Materials and Method: This research was a cross-sectional study. Totally, 60 water samples were collected from five public swimming pools in Kermanshah, Iran. Microbial (parasitec and bacterial) and physicochemical conditions were examined according to the standard method. Results: Results indicated that the average of physicochemical parameters, except temperature, in all women's swimming pools was more sufficient than the men's swimming pools; but, there was no significant difference between them (P > 0.05). Conclusion: It can be said that, gender cannot be very effective (especially, in terms of physiological characteristics of skin) in varying physicochemical conditions and biological parameters in swimming pools. The main reason for changing these parameters might be attributed to some factors, such as type and extent of relationship between physicochemical and biological parameters, characteristics and source of water, type of filtration system, pools' operation and maintenance, effective disinfection, personal hygiene, etc.